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2023-03-09T02:38:14Z

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2023-02-23T21:54:00Z

AshleyOgden533:

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2023-02-23T21:33:20Z

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2023-02-23T21:24:09Z

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Hall J, Guo G, Wray J, Eyres I, Nichols J, Grotewold L, et al. We present that the E3 ubiquitin ligase adaptor protein Klhl13 promotes pluripotency component expression, when inhibiting MAPK goal gene expression and differentiation. A lipid-anchored Grb2-binding protein that inbound links FGF-receptor activation to the Ras/MAPK signaling pathway. The mammalian MAPK/ERK pathway displays attributes of a adverse feedback amplifier. Subsequent enrichment of cells with amplified MAPK pathway activity and sequencing of their involved sgRNAs allowed identification of genes performing as MAPK inhibitors that, when deleted, elevated MAPK signaling. Identification of X-chromosomal MAPK regulators by way of a pooled CRISPR knockout display screen. A major chromosome-large CRISPR knockout monitor and a few secondary screens assaying for distinct factors of the feminine pluripotency phenotype let us to uncover numerous genes that act in concert and to disentangle their relative roles. 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2023-02-17T20:59:55Z

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2023-02-17T20:41:59Z

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2023-02-12T20:55:44Z

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2023-02-06T20:26:31Z

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2023-02-05T04:20:05Z

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